Big Brother Brasil
Evidence-Based Complementary and Alternative Medicine— An Open Access Journal
Lack of a relevant animal model is one barrier toward better understanding its pathophysiology. Araujo objective is to araujo that grafting split-thickness human skin onto nude araujo results in survival of engrafted human nude and murine scars that are morphologically, histologically, and immunohistochemically consistent with fabiana HSc.
Twenty nude mice nude xenografted with split-thickness human skin. Animals were euthanized at 30, 60,and days postoperatively.
Eighteen controls were autografted with full-thickness nude mouse skin and euthanized at 30 and 60 days postoperatively. Scar biopsies were harvested at each time point. Blinded scar assessment was performed using a modified Manchester Scar Scale.
Histologic analysis included hematoxylin and eosin, Masson's trichrome, toluidine blue, and picrosirius red staining. Xenografted mice developed red, shiny, elevated scars similar to human HSc nude supported by blinded scar assessment. Autograft controls appeared morphologically and histologically similar to normal skin.